项目名称： 高压静电场对甘草植物的表观遗传效应及其机制研究

项目编号： No.51467014

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 电工技术

项目作者： 郭九峰

作者单位： 内蒙古大学

项目金额： 48万元

中文摘要： 形态和生理水平上对电磁场的生物效应研究取得了很大的成效。随着研究和应用的不断深入，一些新的研究热点涌现出来,电磁场的表观遗传效应就是其中之一。表观遗传修饰，广泛存在于动植物基因组中，在维持基因组稳定性、防御外来基因入侵、抵御不良环境胁迫、调节基因表达等方面起着重要作用。特殊的环境条件刺激能引起植物的表观遗传变异,是目前植物研究的热点之一。已有证据表明电场条件下植物的变化符合表观遗传变异特点，但这方面研究尚鲜有报道，为探究高压电场对植物的表观遗传效应，本项目以甘草为材料，通过不同形式的电场处理，采用高效液相色谱技术（HPLC）和甲基化敏感扩增多态性技术(MSAP)对电场处理前后的种子、幼苗及不同生育期的甘草植株DNA信息进行提取，基于植物DNA甲基化对电磁表观遗传效应进行研究，从根本上揭示电磁场在植物上的生物效应，为创新电磁生物信息提取技术，拓宽电磁装置在植物上的应用，提供理论和实践支撑。

中文关键词： 电磁场；生物效应；表观遗传；甘草；植物

英文摘要： Great achievements of electromagnetic fields biological effects has made on Morphological and physiological studies.With the research and application of the electric field biological effect getting deeper, some new research hotspots appeared. The epigenetic effects of electromagnetic field is one case.epigenetic modification is widely exists in animals and plants' genome. It plays an important role in maintenance of genomic stability, defense of foreign genes invasion, environmental stress resist and genetic expression regulate.Because plant DNA methylation increases the information amount of the genome, it is one of the research focuses. However, the methylation under the electric field hardly has paper till now.To explore how the high-voltage electric field affects licorice DNA methylation and character variation, this study use high-performance liquid chromatography (HPLC) and Methylation Sensitive Amplification Polymorphism (MSAP) to deal with liquorices' plants in different electric field processing and growth periods. Their DNA methylation level and methylation modification polymorphism loci were determined by sequence analysis and clone and were functional annotation through homologous sequences which were matched.This effects study of electromagnetic epigenetic inheritance, which is based on plant DNA methylation, would make innovations in the extraction technology of electromagnetic biological information. In summary, the change of Licorice DNA methylation in the high-voltage prick electrostatic field could reveal the biological effect of electromagnetic field, broaden the application field of electromagnetic devices and provide new ideas and theoretical guidance for the application of the electromagnetic field on plant.

英文关键词： high voltage electrostatic field;biological effects;epigenetic;licorice;plant