Anomaly segmentation plays a crucial role in identifying anomalous objects within images, which facilitates the detection of road anomalies for autonomous driving. Although existing methods have shown impressive results in anomaly segmentation using synthetic training data, the domain discrepancies between synthetic training data and real test data are often neglected. To address this issue, the Multi-Granularity Cross-Domain Alignment (MGCDA) framework is proposed for anomaly segmentation in complex driving environments. It uniquely combines a new Multi-source Domain Adversarial Training (MDAT) module and a novel Cross-domain Anomaly-aware Contrastive Learning (CACL) method to boost the generality of the model, seamlessly integrating multi-domain data at both scene and sample levels. Multi-source domain adversarial loss and a dynamic label smoothing strategy are integrated into the MDAT module to facilitate the acquisition of domain-invariant features at the scene level, through adversarial training across multiple stages. CACL aligns sample-level representations with contrastive loss on cross-domain data, which utilizes an anomaly-aware sampling strategy to efficiently sample hard samples and anchors. The proposed framework has decent properties of parameter-free during the inference stage and is compatible with other anomaly segmentation networks. Experimental conducted on Fishyscapes and RoadAnomaly datasets demonstrate that the proposed framework achieves state-of-the-art performance.

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