Although the Cassini Spacecraft and the Huygens lander provided numerous information about Titan atmospheric chemistry and the formation of its aerosols, the exact composition of these aerosols still remains unknown. A fruitful proxy to investigate these aerosols is the use of laboratory experiments that allow producing and studying analogs of Titan aerosol, the so35 called tholins. Even when produced in the laboratory, unveiling the exact composition of the aerosol remains problematic due to the high complexity of the material. Numerous advances have been recently made using high-resolution mass spectrometry (HRMS) (Pernot et al. 2010, Somogyi et al. 2012, Gautier et al. 2014) that allowed the separation of isobaric compounds and a robust identification of chemical species composing tholins regarding their molecular formulae. Nevertheless isomeric species cannot be resolved by a simple mass measurement. We propose here an analysis of tholins by high performance liquid chromatography (HPLC) coupled to HRMS to unveil this isomeric ambiguity for some of the major tholins compounds. By comparing chromatograms obtained when analyzing tholins and chemical standards, we strictly identified seven molecules in our tholins samples: melamine, cyanoguanidine, 6-methyl-1,3,5-triazine-2,4-diamine, 2,4,6-triaminopyrimidine, 3-amino- 1,2,4-triazole, 3,5-Dimethyl-1,2,4-triazole and 2,4-diamino-1,3,5-triazine. Several molecules, including hexamethylenetriamine (HMT) were not present at detectable levels in our sample. The use for the first time of a coupled HPLC-HRMS technique applied to tholins study demonstrated the interest of such a technique compared to single high-resolution mass spectrometry for the study of tholins composition.

翻译：虽然Cassini Spacecraft和Huygens登陆器提供了大量有关泰坦大气化学及其气溶胶形成的信息,但这些气溶胶的确切成分仍然未知。调查这些气溶胶的有益替代物是使用实验室实验,能够生产和研究Titan气溶胶的模拟物,这叫Tholins。即使是在实验室生产的,暴露气溶胶的确切成分仍然有问题,因为材料的高度复杂性。最近利用高分辨率质谱测量(Pernot等人,2010年;Somogyi等人,2012年;Gautier等人,2014年)来调查这些气溶胶的准确成分,从而得以分离异baric化合物,并强有力地识别含有分子配方的化学物种。然而,我们在这里建议通过高性液相色谱分析来分析硫柳酯的准确成分。 与HRMS一起将这种异狄里亚基离子离子质质测量(Periorialiyalimus-deal-alimayal-deal-deal-alimayal mayal) 6,我们在分析Hthtrial-drial-drial-drial-drialexmexmex mex mexmexmexmex mex mex mex mex mex mex mex mex mex 和化学标准时,在7-mo-mo-de-mo-mo-mo-moxx 上发现了一种高值中首次的兴趣。