With the development of IoT and edge computing, there is a need for efficient and reliable middleware to handle the communication among Edge devices or between Edge and Cloud. Meanwhile, ROS\,2 is more commonly used in robotic systems, but there is no comparison study of middleware using ROS Messages. In this study, we compared the middlewares that are commonly used in ROS\,2 systems, including DDS, Zenoh, and MQTT. In order to evaluate the performance of the middleware in Edge-to-Edge and Edge-to-Cloud scenarios, we conducted the experiments in a multi-host environment and compared the latency and throughput of the middlewares with different types and sizes of ROS Messages in three network setups including Ethernet, Wi-Fi, and 4G. Additionally, we implemented different middlewares on a real robot platform, TurtleBot 4, and sent commands from a host to the robot to run a square-shaped trajectory. With the Optitrack Motion Capture system, we recorded the robot trajectories and analyzed the drift error. The results showed that CycloneDDS performs better under Ethernet, and Zenoh performs better under Wifi and 4G. In the actual robot test, Zenoh's trajectory drift error was the smallest.

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